Benchmarking Non-Targeted Metabolomics Using Yeast-Derived Libraries

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isotopologue ratio normalization for non-targeted metabolomics.

Robust quantification of analytes is a prerequisite for meaningful metabolomics experiments. In non-targeted metabolomics it is still hard to compare measurements across multiple batches or instruments. For targeted analyses isotope dilution mass spectrometry is used to provide a robust normalization reference. Here, we present an approach that allows for the automated semi-quantification of me...

متن کامل

Metabolic engineering of a tyrosine-overproducing yeast platform using targeted metabolomics

BACKGROUND L-tyrosine is a common precursor for a wide range of valuable secondary metabolites, including benzylisoquinoline alkaloids (BIAs) and many polyketides. An industrially tractable yeast strain optimized for production of L-tyrosine could serve as a platform for the development of BIA and polyketide cell factories. This study applied a targeted metabolomics approach to evaluate metabol...

متن کامل

Method validation strategies involved in non-targeted metabolomics.

Non-targeted metabolomics is the hypothesis generating, global unbiased analysis of all the small-molecule metabolites present within a biological system, under a given set of conditions. It includes several common steps such as selection of biological samples, sample pre-treatment, analytical conditions set-up, acquiring data, data analysis by chemometrics, database search and biological inter...

متن کامل

Targeted metabolomics for bioprocessing

Background Bioprocesses like the cell-based production of biologicals, i.e. mainly recombinant proteins and monoclonal antibodies, require optimal culture conditions to obtain a high yield of quality products. The performance of a bioreactor highly depends on the cell characteristics as well as on the composition of the cell culture medium and the process conditions. As the metabolic activity o...

متن کامل

Impact of statistical models on the prediction of type 2 diabetes using non-targeted metabolomics profiling

OBJECTIVE Characterizing specific metabolites in sub-clinical phases preceding the onset of type 2 diabetes to enable efficient preventive and personalized interventions. RESEARCH DESIGN AND METHODS We developed predictive models of type 2 diabetes using two strategies. One strategy focused on the probability of incidence only and was based on logistic regression (MRS1); the other strategy ac...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Metabolites

سال: 2021

ISSN: 2218-1989

DOI: 10.3390/metabo11030160